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南昌大学何庆华研究员团队‖【Anal.Chem. 】模拟表位驱动的lucCage−lucKey均相生物发光免疫传感器用于小分子伏马菌素B1的检测

  • 2026-06-29 15:52:44
南昌大学何庆华研究员团队‖【Anal.Chem. 】模拟表位驱动的lucCage−lucKey均相生物发光免疫传感器用于小分子伏马菌素B1的检测

少一点试错

多一点突破

介绍 /Introduction

伏马毒素B1（Fumonisin B1，FB1）是一类常见的小分子真菌毒素，广泛污染玉米、小麦等粮食及其制品，长期摄入会对食品安全和人体健康造成潜在威胁。因此，建立灵敏、快速、便捷的小分子毒素检测方法具有重要意义。均相免疫分析具有操作简单、无需洗涤、灵敏度高、自动化潜力强等优势，是快速检测领域的重要发展方向。然而，小分子化合物由于结构简单、缺乏多个结合表位，难以像大分子抗原一样直接触发显著的信号变化；同时，传统均相免疫传感器往往依赖化学偶联、抗体改造或重新开发特异性识别元件，存在构建复杂、成功率不稳定、成本较高等问题。
针对这一瓶颈，近日，南昌大学中德联合研究院、食品科学与资源挖掘全国重点实验室何庆华研究员团队提出了一种由模拟表位（mimotope）驱动的lucCage-lucKey 均相生物发光免疫传感策略。研究团队以伏马毒素 B1为模型靶标，将可模拟FB1抗原表位并竞争结合抗FB1单克隆抗体的短肽F1，作为识别模块引入 lucCage 蛋白开关系统中。通过 Rosetta 计算设计与AlphaFold3 结构预测，团队构建了两类FB1均相生物发光传感器：模拟表位单端嫁接型和双端融合型lucCage-lucKey 传感器。该策略无需改造现有抗体，也无需重新制备新抗体，可直接利用已有小分子单克隆抗体资源，实现完全生物合成的均相免疫检测体系构建。
相关成果以 “ Mimotope-Driven lucCage-lucKey homogeneous bioluminescent immunosensor for small molecule Fumonisin B1” 为题发表在《Anal. Chem.》。

研究亮点

1.将lucCage–lucKey蛋白开关拓展用于小分子的均相检测。

2.建立了无需改造单克隆抗体的小分子均相免疫分析方法。

3.模块化的模拟表位嫁接策略使该平台可便捷适配于其他小分子靶标。

4.检测体系中所有关键元件均可通过生物合成方式制备，构建了全基因编码的绿色检测流程。

「全文速递」南昌大学何庆华研究员团队‖Anal.Chem.模拟表位驱动的lucCage−lucKey均相生物发光免疫传感器用于小分子伏马菌素B1的检测.pdf

研究图文/Introduction

图 1. 模拟表位单端嫁接型lucCage 传感器（a）和双端融合模拟表位型lucCage 传感器（b）的作用机制示意图。

图 2. 模拟表位单端嫁接型 lucCage传感器的设计与筛选。

图3. 模拟表位单端嫁接型lucCage−lucKey 传感器用于FB1 检测。

图 4. 模拟表位双端融合型lucCage 传感器的开发。
图5. 模拟表位双端融合型 lucCage 传感器的优化。

结论 /Conclusion

本研究构建了一种基于模拟表位和lucCage-lucKey 蛋白开关驱动的均相生物发光免疫传感平台，实现了小分子真菌毒素 FB1的快速、灵敏、均相免疫检测。研究团队最终获得了两类性能良好的 FB1 均相生物发光传感器。其中，基于模拟表位单端嫁接型优化传感器Cb-lucCageF1_342的检测限达到了2.99 ng/mL，在实际样品检测中，FB₁在玉米和小麦基质中的检测限分别为44.91 μg/kg和30.02 μg/kg，加标回收率分别为81.27%–118.43%和92.15%–124.71%；基于模拟表位双端融合型优化传感器 NCb-lucCageF1_S_L2的检测限为9.73 ng/mL，对FB1检测灵敏度相较于优化前提高了 1.7 倍。两类传感器均表现出良好特异性，对 AFB1、DON、OTA 和ZEN 等常见真菌毒素无明显交叉反应。

总体而言，本研究为小分子均相免疫分析提供了一种新的蛋白工程设计路径，也为食品安全、环境监测及体外诊断领域中小分子污染物的快速检测提供了可拓展的技术平台。

文献直通车 /Literature information

Mimotope-Driven lucCage-lucKey Homogeneous Bioluminescent Immunosensor for the Small Molecule Fumonisin B1

Binhui Huang, Minwen Sun, Xuhong Liu, Chong Cai, Tailong Wei, Meijuan Huang, Zhui Tu, Yanping Li, and Qinghua He*

原文链接：https://doi.org/10.1021/acs.analchem.6c01299

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  1. CONNECT:[ UseTime:0.000749s ] mysql:host=127.0.0.1;port=3306;dbname=aa460;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `fenlei` [ RunTime:0.000661s ]
  3. SELECT * FROM `fenlei` WHERE `fid` = 0 [ RunTime:0.000816s ]
  4. SELECT * FROM `fenlei` WHERE `fid` = 63 [ RunTime:0.001494s ]
  5. SHOW FULL COLUMNS FROM `set` [ RunTime:0.000533s ]
  6. SELECT * FROM `set` [ RunTime:0.000881s ]
  7. SHOW FULL COLUMNS FROM `article` [ RunTime:0.000579s ]
  8. SELECT * FROM `article` WHERE `id` = 532488 LIMIT 1 [ RunTime:0.010621s ]
  9. UPDATE `article` SET `lasttime` = 1782771412 WHERE `id` = 532488 [ RunTime:0.004301s ]
  10. SELECT * FROM `fenlei` WHERE `id` = 64 LIMIT 1 [ RunTime:0.000886s ]
  11. SELECT * FROM `article` WHERE `id` < 532488 ORDER BY `id` DESC LIMIT 1 [ RunTime:0.001657s ]
  12. SELECT * FROM `article` WHERE `id` > 532488 ORDER BY `id` ASC LIMIT 1 [ RunTime:0.010804s ]
  13. SELECT * FROM `article` WHERE `id` < 532488 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.006528s ]
  14. SELECT * FROM `article` WHERE `id` < 532488 ORDER BY `id` DESC LIMIT 10,10 [ RunTime:0.002296s ]
  15. SELECT * FROM `article` WHERE `id` < 532488 ORDER BY `id` DESC LIMIT 20,10 [ RunTime:0.001980s ]
0.206331s